在高等真核生物中，很多基因的前体 mRNA 含有内含子，表达时需要切除内含子连接外显子。行使这种剪接功能的是一类核酸蛋白复合体，被称为剪接体。前体mRNA剪接是真核细胞基因表达调控的基本机制，研究mRNA剪接的分子机制，能促进对真核基因表达调控机制的认识。
最近，实验室在稻瘟菌信号通路及致病相关基因功能研究方面又取得了新进展，揭示了硫还原氧化蛋白通过激活 PMK1 MAPK 信号通路调控稻瘟菌侵染过程的致病机理。
Adenosine Deaminase Acting on RNA (ADAR) enzymes mediated adenosine-to-inosine (A-to-I) editing is the most prevalent post-transcriptional modification of RNA in animals. Organisms outside animal kingdom do not have ADAR orthologs and are believed to lack A-to-I RNA editing. However, we demonstrates genome-wide A-to-I RNA editing occurs in fungi specifically during sexual reproduction, involving adenosine deamination mechanisms distinct from animal ADARs. Genome-wide analysis identified 26,056 perithecium-specific A-to-I editing sites in Fusarium graminearum. Unlike those in animals, 70.5% of A-to-I editing sites in F. graminearum occur in coding regions, and more than two-thirds of them result in amino acid changes, including editing of 70 pseudogenes with stop codons in ORFs. F. graminearum differs from animals in the sequence preference and structure selectivity of A-to-I editing sites. Whereas A’s embedded in RNA stems are targeted by ADARs, RNA editing in F. graminearum preferentially targets A’s in hairpin loops, which is similar to the anticodon loop of tRNA targeted by adenosine deaminases acting on tRNA (ADATs). In addition, we also identified A-to-I RNA editing events in Neurospora crassa and F. verticillioides.
Recently, we demonstrated a new mechanism in DON regulation, revealed the relationship between cAMP-mediated signaling and DON biosynthesis in Fusarium graminearum. Fusarium head blight (FHB) caused by F. graminearum is a major threat to global wheat production. In addition to severe yield losses and grain quality reduction, F. graminearum produces trichothecene mycotoxin deoxynivalenol (DON) and other toxic secondary metabolites in infested grains. Thus, it is very important to study the mechanism of DON regulation in order to prevent fusarium head blight. In this study, researchers showed that cAMP treatment induced DON production by stimulating TRI gene expression and DON-associated cellular differentiation in F. graminearum. Although the two cAMP phosphodiesterase genes PDE1 and PDE2 had overlapping functions in vegetative growth, conidiation, sexual reproduction, and plant infection, Deletion of PDE2 but not PDE1 activated intracellular PKA activities and increased DON production. They also revealed that TRI6 was essential for the regulation of DON biosynthesis by cAMP signaling but elevated PKA activities could partially bypass the requirement of TRI10 for TRI gene-expression and DON production, and Pde2 was the major cAMP phosphodiesterase to negatively regulate DON biosynthesis in F. graminearum.